The T!Raum One-Health initiative of the University of Greifswald, funded by the German Federal Ministry of Education and Research, aims to operationalise the One Health approach in Western Pomerania. For example, T!Raum supports health-transforming R&D-, citizen science-, and fundamental research projects, connects research, economy, politics and other health actors, and engages with local communities through participatory formats. Multiple regional partner institutions and networks from various hierarchical societal levels and health sectors support and engage in T!Raum.