Pan African Tsetse and Trypanosomiasis Eradication Campaign-Nigeria is a project managed by the Nigerian Institute for Trypanosomiasis and Onchocerciasis Research (NITR). Human African trypanosomiasis is a zoonotic disease with one health implications. Recently, few transmission hotspots have been identified in Nigeria underpinning resurgence threat. Our One Health event targets the 200+ NITR research staff, government officials, health-related industries, participants from other institutions, and members of professional societies across the globe.