The II One Health Workshop / UFMS aims to bring together professionals, students and anyone interested in One Health approach, which is the multidisciplinary approach to health, considering the inseparable human-animal-environment pillars. This event will address emerging arboviruses, the role of migratory birds as reservoirs for exotic diseases, re-emergence of tuberculosis and human brucellosis therapy, now available through the Brazilian public health system (SUS).